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Competence for callus induction on mature pepper leaves depends upon specific developmental stages of the donor plant

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dc.contributor.author Kintzios, S en
dc.contributor.author Drossopoulos, J en
dc.contributor.author Manousaridou, M en
dc.contributor.author Holevas, CD en
dc.date.accessioned 2014-06-06T06:43:09Z
dc.date.available 2014-06-06T06:43:09Z
dc.date.issued 1996 en
dc.identifier.issn 03044238 en
dc.identifier.uri http://dx.doi.org/10.1016/0304-4238(96)00885-0 en
dc.identifier.uri http://62.217.125.90/xmlui/handle/123456789/1052
dc.subject Callus competence en
dc.subject Capsicum annuum L. en
dc.subject Cell habituation en
dc.subject Culture response en
dc.subject Developmental stage en
dc.subject Threshold sensitivity en
dc.title Competence for callus induction on mature pepper leaves depends upon specific developmental stages of the donor plant en
heal.type journalArticle en
heal.identifier.primary 10.1016/0304-4238(96)00885-0 en
heal.publicationDate 1996 en
heal.abstract Mature leaf explants of two pepper (Capsicum annuum L.) cultivars, 'Edino' and 'Brujo', were cultured on Murashige and Skoog medium supplemented with 12.9 μM 6-benzyladenine and either 4.9 μM indolebutyric acid or 13.6 μM 2,4-dichlorophenoxyacetic acid. Callus induction depended very significantly upon the position of the leaves on the donor plants. Callus tissue induced on responding explants showed two distinct morphological types which were further subcultured on induction media or on Murashige and Skoog medium supplemented with either 2.6 μM 6-benzyladenine and 2.5 μM indolebutyric acid or 25.8 μM 6-benzyladenine and 9.8 μM indolebutyric acid. Proliferation of subcultured callus tissue depended significantly on callus morphology, genotype, developmental stage and the type and concentration of auxin used. The possible relationship of the observed results to callus habituation in culture is discussed. en
heal.journalName Scientia Horticulturae en
dc.identifier.issue 4 en
dc.identifier.volume 65 en
dc.identifier.doi 10.1016/0304-4238(96)00885-0 en
dc.identifier.spage 341 en
dc.identifier.epage 347 en


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